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Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Renmin Feng, Lei Sun, Ailing Kong, Lingyi |
| Copyright Year | 2004 |
| Abstract | A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of coumarins from Peucedanum praeruptorum Dunn (Baihuaqianhu in Chinese) was successfully established by using light petroleum-ethyl acetate-methanol-water as the two-phase solvent system in gradient elution mode. The upper phase of light petroleum-ethyl acetate-methanol-water (5:5:5:5, v/v) was used as the stationary phase of HSCCC. The mobile phase used in HSCCC was the lower phase of light petroleum-ethyl acetate-methanol-water (5:5:5:5, v/v) and light petroleum-ethyl acetate-methanol-water (5:5:6.5:3.5, v/v) that was changed in gradient. Four kinds of coumarins and another unknown compound were obtained and yielded 5.3 mg of qianhucoumarin D, 7.7 mg of Pd-Ib, 35.8 mg of (+)-praeruptorin A, 31.9 mg of (+)-praeruptorin B and 6.4 mg of unknown compound with the purity of 98.6%, 92.8%, 99.5%, 99.4% and 99.8% in one-step separation, respectively. The structures of the coumarins were identified by 1H NMR and 13C NMR. |
| Starting Page | 89 |
| Ending Page | 94 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.chroma.2004.09.047 |
| PubMed reference number | 15584226 |
| Journal | Medline |
| Volume Number | 1057 |
| Issue Number | 1-2 |
| Alternate Webpage(s) | http://tautobiotech.com/en/download/application%20of%20hsccc/field%20of%20tcm/preparation%20and%20separation/coumarin%20,essential%20oil,%20lactone/preparative%20isolation%20and%20purification%20of%20coumarins%20from%20peucedanum%20praeruptorum%20dunn.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.chroma.2004.09.047 |
| Journal | Journal of chromatography. A |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
