Preparative isolation and purification of costunolide and dehydrocostuslactone from Aucklandia lappa Decne by high-speed counter-current chromatography.

Content Provider	Semantic Scholar
Author	Li, Aifeng Sun, Ailing Liu, Renmin
Copyright Year	2005
Abstract	A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of costunolide and dehydrocostuslactone from the Chinese medicinal plant Aucklandia lappa Decne (Muxiang in Chinese) was successfully established by using light petroleum-methanol-water (5:6.5:3.5, v/v/v) as the two-phase solvent system. The upper phase of light petroleum-methanol-water (5:6.5:3.5, v/v/v) was used as the stationary phase of HSCCC. 35.7 mg of costunolide and 43.6 mg of dehydrocostuslactone with the purity of 100% and 99.6%, respectively, were separated successfully in one-step separation from 110 mg of crude sample from Aucklandia lappa Decne. The structures of costunolide and dehydrocostuslactone were identified by 1H NMR and 13C NMR.
File Format	PDF HTM / HTML
PubMed reference number	15974088
Journal	Medline
Volume Number	1076
Issue Number	1-2
Alternate Webpage(s)	http://www.tautobiotech.com/en/download/application%20of%20HSCCC/field%20of%20TCM/preparation%20and%20separation/coumarin%20,essential%20oil,%20lactone/Preparative%20isolation%20and%20purification%20of%20costunolide%20and%20dehydrocostuslactone.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.chroma.2005.04.042
Journal	Journal of chromatography. A
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article