NDLI: Preparative isolation and purification of syringin and edgeworoside C from Edgeworthia chrysantha Lindl by high-speed counter-current chromatography.

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Preparative isolation and purification of syringin and edgeworoside C from Edgeworthia chrysantha Lindl by high-speed counter-current chromatography.

Content Provider	Semantic Scholar
Author	Yan, Jizhong Tong, Shengqiang Chu, Jianjun Sheng, Liuqing Chen, Gang
Copyright Year	2004
Abstract	The bioactive compound syringin along with edgeworoside C were separated from the n-butanol extract of the stems and barks of Edgeworthia chrysantha Lindl (E. papyrifera) by high-speed counter-current chromatography (HSCCC) while it was difficult to purify each compound by silica gel column chromatography. Syringin was isolated from this plant for the first time. The two-phase solvent system used was composed of ethyl acetate-ethanol-water at an optimized volume ratio of 15:1:15 (v/v/v). Preparative HSCCC yielded, from 110mg of the partially purified extract, 28mg of syringin and 45 mg edgeworoside C each at over 96% purity by high-performance liquid chromatography analysis. Their structures were identified by electron impact ionization MS, 1H NMR and 13C NMR.
File Format	PDF HTM / HTML
DOI	10.1016/j.chroma.2004.05.087
PubMed reference number	15330108
Journal	Medline
Volume Number	1043
Issue Number	2
Alternate Webpage(s)	http://www.tautobiotech.com/en/download/application%20of%20HSCCC/field%20of%20TCM/preparation%20and%20separation/indicans/Preparative%20isolation%20and%20purification%20of%20syringin%20and%20edgeworoside%20C%20from.pdf
Journal	Journal of chromatography. A
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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