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3,3', 5,5'-tetramethylbenzidine as electrochemical substrate for horseradish peroxidase based enzyme immunoassays. A comparative study
| Content Provider | Semantic Scholar |
|---|---|
| Author | Volpe, G. Draisci, Rosa Palleschi, Giuseppe Compagnone, Dario |
| Copyright Year | 1998 |
| Abstract | The use of 3,3′,5,5′-tetramethylbenzidine (TMB) as an electrochemical substrate for horseradish peroxidase (HRP) was investigated. HRP activity has been detected using flow injection analysis at a glassy carbon working electrode polarised at +100 mV versus Ag/AgCl in 0.1 mol l–1 citrate–phosphate buffer (pH 5.0). The optimum concentrations were 2 × 10–4 mol l–1 TMB and 10–3 mol l–1 H2O2. The detection limit obtained after 15 min of incubation was 8.5 × 10–14 mol l–1 HRP with the amperometric method. This limit was lower than that obtained using hydroquinone as HRP substrate and comparable to that with the p-aminophenyl phosphate–alkaline phosphatase system. Better performance was achieved with amperometric than spectrophotometric detection using TMB in a competitive ELISA for rabbit immunoglobulin G as a model analyte. |
| Starting Page | 1303 |
| Ending Page | 1307 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/A800255J |
| Volume Number | 123 |
| Alternate Webpage(s) | http://www.uniroma2.it/dipartim/BEAT/pubblicazioni/2.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/A800255J |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
