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3,3',5,5'-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Busa, Lori Shayne Alamo Komatsu, Takeshi Mohammadi, Saeed Maeki, Masatoshi Ishida, Akihiko Tani, Hirofumi Tokeshi, Manabu |
| Copyright Year | 2016 |
| Abstract | We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration. |
| File Format | PDF HTM / HTML |
| DOI | 10.2116/analsci.32.815 |
| PubMed reference number | 27506705 |
| Journal | Medline |
| Volume Number | 32 |
| Issue Number | 8 |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/analsci/32/8/32_815/_pdf/-char/en |
| Journal | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |