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Increases in the density of parvalbumin-immunoreactive neurons in anterior cingulate cortex of amphetamine-withdrawn rats: evidence for corticotropin-releasing factor in sustained elevation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mohila, Carrie A. Onn, Shao-Pii |
| Copyright Year | 2005 |
| Abstract | We previously reported synchronization of pyramidal neurons within prefrontal cortex of rats repeatedly exposed to amphetamine (AMPH). To test the hypothesis that cortical synchronization may be related to changes in local GABA signaling, we used antibodies specific for parvalbumin (PV), calbindin D28k (CB) and calretinin (CR) as selective labels for three distinct GABA interneuron classes in the anterior cingulate cortex (ACC) of similarly treated rats. We observed a selective increase in the density of PV-immunoreactive (ir), but not CB-ir or CR-ir, neurons in the ACC of AMPH-treated rats at both 1 day and 7 day withdrawal. Increased density of PV-ir GABA interneurons in the ACC at 1 day withdrawal was reproduced in rats repeatedly injected with apomorphine or with SKF-38393. Thus, the critical role of DA receptors during AMPH exposure is evident. However, DA receptor activation did not appear to account for the PV up-regulation in AMPH-treated rats at 7 day withdrawal. Significantly higher numbers of pericellular basket-like puncta immunoreactive for corticotropin-releasing factor (CRF) were observed in the ACC of AMPH rats at 7 day withdrawal. Combined dual immunofluorescence and confocal microscopy further revealed that CRF-ir puncta made possible pericellular contacts on PV-ir (not CB-, CR- or glutamate-ir) cell bodies. A potential cellular mechanism seems to emerge that CRF-ir terminals, that may be underdetected under normal conditions due to low activity levels, may be functionally activated during psychostimulant withdrawal, thereby altering local GABAergic signaling. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://cercor.oxfordjournals.org/content/15/3/262.full.pdf |
| PubMed reference number | 15238432v1 |
| Volume Number | 15 |
| Issue Number | 3 |
| Journal | Cerebral cortex |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | AMPH gene AMPH protein, human Amphetamine Apomorphine Calbindins Cerebral cortex Cingulate Cortex Class Corticotropin-Releasing Hormone DSIP-immunoreactive peptide Dopamine Dual Fluorescent Antibody Technique Glutamic Acid Gonadorelin Gray matter of anterior cingulate gyrus Interneurons Microscopy, Confocal PVALB gene Parvalbumins Penicillin V Prefrontal Cortex Pyramidal Cells Receptor Activation Process SK&F-38393 Up-Regulation (Physiology) calbindin 1 calretinin cell body gamma-Aminobutyric Acid pericellular basket |
| Content Type | Text |
| Resource Type | Article |
