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Overexpression of Long Non-Coding RNA ZXF2 Promotes Lung Adenocarcinoma Progression Through c-Myc Pathway.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yang, Ze-Tian Li, Zhihong Wang, Xian-guo Tan, Tao Yi, Frank Zhu, Hua Zhao, Jin-ping Zhou, Xue-feng |
| Copyright Year | 2015 |
| Abstract | OBJECTIVE To investigate the expression of long non-coding RNA ZXF2 in lung adenocarcinoma tissues and its effect on cell proliferation, migration and invasion. METHODS Forty pairs of cancerous and adjacent non-cancerous lung adenocarcinoma specimens were collected for the studies. Quantitative real-time PCR was used to analyze the expression of ZXF2 in tumor tissues and adjacent normal tissues. The expression of ZXF2 was correlated with patients' clinico-pathological data. Molecular pathway controlled by ZXF2 was explored by using small interfering RNA (siRNA) technology. CCK-8 cell proliferation assay, flow cytometry analysis and transwell assays were used to evaluate cell proliferation, migration and invasion. RESULTS The expression of ZXF2 was 2 fold or higher in 27 out of 40 (67.5%) cases of lung adenocarcinoma specimens than that in non-cancerous tissues (P<0.05). The relative expression level of ZXF2 was positively correlated with tumor lymph node metastasis (χ(2)=8.485, P<0.05) and poor prognosis of the patients (p=0.0217). In order to explore the molecular mechanisms of ZXF2 mediated tumor progression, ZXF2 expression was inhibited by siRNA in A549 cells, a highly aggressive and metastatic lung adenocarcinoma cell line. We found that siRNA-ZXF2 treatment inhibited cell proliferation (P<0.01) leading to cell cycle arrest (P<0.01). The cell migration and invasion were suppressed by siRNA-ZXF2 treatment (P<0.01). Further biochemical studies revealed that the knockdown of ZXF2 led to down regulation of c-Myc signaling. CONCLUSION ZXF2 was overexpressed in lung adenocarcinoma tissues and the high expression of ZXF was closely related to tumor progression through c-Myc related pathway. Given the fact that both ZXF2 and c-Myc are located in the same chromosome 8q24.2 loci, the potential interaction between ZXF2 and c-Myc might be a novel target for treatment of lung adenocarcinoma. |
| Starting Page | 153 |
| Ending Page | 157 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.karger.com/Article/Pdf/374038 |
| PubMed reference number | 25896422v1 |
| Alternate Webpage(s) | https://doi.org/10.1159/000374038 |
| DOI | 10.1159/000374038 |
| Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology |
| Volume Number | 35 |
| Issue Number | 6 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 8q24.2 Adenocarcinoma of lung (disorder) Anatomic Node Antigens Body tissue Cell Cycle Arrest Cell Proliferation Assay Digital Object Identifier Down-Regulation Flow Cytometry Lung diseases MYC gene Matticnemis doi Natural Science Disciplines Neoplasms Non-Small Cell Lung Carcinoma Patients RNA, Small Interfering Scientific Publication Sincalide Specimen Stage IV Lung Adenocarcinoma Tumor Progression cancer cell conversion of ds siRNA to ss siRNA involved in chromatin silencing by small RNA lymph node metastases lymph nodes non-T, non-B childhood acute lymphoblastic leukemia |
| Content Type | Text |
| Resource Type | Article |
