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Long non-coding RNA H19 promotes the proliferation of fibroblasts in keloid scarring.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Jie Liu, Cai Yue Wan, Yun Peng, Li Li, Wen Fang Qiu, Jia |
| Copyright Year | 2016 |
| Abstract | The expression of the long non-coding RNA (lncRNA) H19 is associated with proliferation in tumors. In order to investigate whether H19 may additionally mediate the proliferation of fibroblasts in human keloid disease, the present study collected samples from 24 subjects, including 8 with keloids, 8 with normal scars and 8 normal skin controls. Reverse transcription-polymerase chain reaction revealed that H19 levels were markedly increased in human keloids compared with normal scars and normal skin controls (P=0.017). In order to identify a potential role for H19 in the proliferative activity of human keloid fibroblasts, small interfering (si)RNA-mediated silencing experiments were performed. H19 siRNA treatment markedly inhibited the proliferation of keloid fibroblasts, as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (P=0.008). In order to identify the signaling mediators that are regulated by H19 in keloid fibroblasts, the expression levels of mammalian target of rapamycin (mTOR) and vascular endothelial growth factor (VEGF) were examined using western blotting. The results confirmed that knockdown of H19 inhibited mTOR and VEGF expression. In summary, the results indicate that H19 may be associated with increased proliferative activity of keloid fibroblasts, which may be mediated by mTOR and VEGF. |
| Starting Page | 2835 |
| Ending Page | 2839 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/ol/12/4/2835/download |
| PubMed reference number | 27698867v1 |
| Volume Number | 12 |
| Issue Number | 4 |
| Journal | Oncology letters |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cicatrix Cicatrization Endothelial Growth Factors FRAP1 protein, human Growth Factor H19 gene Keloid Neoplasms Non-Small Cell Lung Carcinoma Polymerase Chain Reaction Proliferation (morphologic abnormality) Reverse Transcription Sirolimus Western Blotting conversion of ds siRNA to ss siRNA involved in chromatin silencing by small RNA non-T, non-B childhood acute lymphoblastic leukemia non-T, non-B, cALLa positive childhood acute lymphoblastic leukemia |
| Content Type | Text |
| Resource Type | Article |
