NDLI: Cloning and nucleotide sequence of the glutamate decarboxylase-encoding gene gadA from Aspergillus oryzae.

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Cloning and nucleotide sequence of the glutamate decarboxylase-encoding gene gadA from Aspergillus oryzae.

Content Provider	Semantic Scholar
Author	Kato, Yuki Kato, Yoko Furukawa, Keiji Hara, Shodo
Copyright Year	2002
Abstract	We cloned a genomic DNA encoding the glutamate decarboxylase (GAD) from Aspergillus oryzae using a 200-bp DNA fragment as the probe. This DNA fragment was amplified by the reverse transcription polymerase chain reaction with mRNA of A. oryzae as the template and degenerate primers designed from the conserved amino acid sequence of Escherichia coli GAD and Arabidopsis thaliana GAD. Nucleotide sequencing analysis showed that the cloned gene (designated gadA) encoded 514 amino acid residues and contained three introns. Southern hybridization showed that the gadA gene was on a 6.0-kb SacI fragment and that there was a single copy in the A. oryzae chromosome. The cloned gene was functional, because one transformant of A. oryzae containing multiple copies of the gadA gene had 10-fold the GAD activity and a 12-fold increase in gamma-aminobutyric acid production compared with the control strain.
Starting Page	1
Ending Page	4
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.jstage.jst.go.jp/article/bbb/66/12/66_12_2600/_pdf/-char/en
PubMed reference number	12596854v1
Volume Number	66
Issue Number	12
Journal	Bioscience, biotechnology, and biochemistry
Language	English
Access Restriction	Open
Subject Keyword	Amino Acid Sequence Amino Acids Aspergillus oryzae Base Sequence Carboxy-Lyases Clinical Use Template Clone Cells Contain (action) Copy (object) GAD1 gene Glutamate Decarboxylase Glutamic Acid Introns Nucleic Acid Hybridization Nucleotides Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Sequence Analysis gamma-Aminobutyric Acid
Content Type	Text
Resource Type	Article

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