NDLI: Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei

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Identification and molecular cloning of glutamate decarboxylase gene from Lactobacillus casei

Content Provider	Semantic Scholar
Author	Tavakoli, Yasaman Esmaeili, Abolghasem Rabbani, Mohammad Urooj
Copyright Year	2015
Abstract	Gamma-amino butyric acid (GABA) possesses several physiological functions such as neurotransmission, induction of hypotension, diuretic and tranquilizer effects. Production of GABA-enriched products by lactic acid bacteria has been a focus of different researches in recent years because of their safety and health-promoting specifities. In this study, glutamate decarboxylase (gad) gene of a local strains Lactobacillus casei was identified and cloned. In order to clone the gad gene from this strain, the PCR was carried out using primers designed based on conserved regions. The PCR product was purified and ligated into PGEM-T vector. Comparison of obtained sequences shows that this fragment codes the pyridoxal 5'-phosphate binding region. This strain could possibly be used for the industrial GABA production and also for development of functional fermented foods. Gad gene manipulation can also either decrease or increase the activity of enzyme in bacteria.
Starting Page	161
Ending Page	165
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://mbrc.shirazu.ac.ir/pdf_3119_dc42355185136b63cefae7955dc1a7ae.html
PubMed reference number	5019208
Volume Number	4
Journal	Molecular biology research communications
Language	English
Access Restriction	Open
Subject Keyword	Bacteria Carboxy-Lyases Clone Cells Cloning, Molecular Cultured Milk Products Diuretics Food GAD1 gene Generalized Anxiety Disorder Glutamate Decarboxylase Interferon Type II Lactic acid Lactobacillus casei Promotion (action) Pyridoxal Phosphate Synaptic Transmission Tranquilizing Agents gamma-Aminobutyric Acid
Content Type	Text
Resource Type	Article

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