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Pattern of expression of glutamic acid decarboxylase mRNA in the developing rat brain.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bond, Rob |
| Copyright Year | 1988 |
| Abstract | The time and pattern of appearance of glutamic acid decarboxylase (glutamate decarboxylase; EC 4.1.1.15) (GAD) mRNA during the development of the rat brain were analyzed. RNA transfer blot analysis of poly(A)+ RNA from whole brain shows that a 3.7-kilobase transcript is the most abundant form of the message from embryonic day 15 (E15) through adulthood. By E15 this form is present at about 50% of its adult abundance relative to other poly(A)+ mRNA species. At birth the abundance is approximately the same as in the adult. In contrast, the enzyme activity level is only 8% of the adult level at birth and takes 3 weeks to reach adult levels. There are qualitative changes in GAD mRNA during development. Several large (7-9 kilobases) transcripts with strong homology to GAD are enriched in early developmental stages but are barely detectable in the adult. A nuclease protection assay shows a developmentally regulated heterogeneity in a coding portion of the mRNA. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/85/9/3231.full.pdf |
| PubMed reference number | 3362872v1 |
| Volume Number | 85 |
| Issue Number | 9 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Carboxy-Lyases GAD1 gene Generalized Anxiety Disorder Glutamate Decarboxylase Glutamic Acid Homologous Gene L-Epicatechin Nuclease Protection Assays RNA Transcript |
| Content Type | Text |
| Resource Type | Article |
