Loading...
Please wait, while we are loading the content...
Similar Documents
Translocation t(14;18) in B cell lymphomas as a cause for defective immunoglobulin production
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jong, Daphne De Voetdijk, B. M. Ommen, Gertjan Jb van Kluin-Nelemans, Johanna C. Beverstock, Geoffrey C. Kluin, Philip M. |
| Copyright Year | 1989 |
| Abstract | Although follicle center cell (FCC) lymphomas represent mature B cells, a considerable percentage do not have detectable Ig production. We have used Southern blotting and the polymerase chain reaction (PCR) to study the involvement of translocations t(14;18) and t(8;14) in causing defective Ig production in 16 Ig- FCC-derived lymphomas and three Ig- B cell acute lymphoblastic leukemias. In 6 of 19 cases, a t(14;18) was present with the other allele either deleted or in germline. In two cases a t(14;18) and a t(8;14) affected both Ig alleles, as confirmed by karyotyping. In two other cases, rearrangement of both bcl-2 on chromosome 18 and c-myc on chromosome 8 were found as well. Although cytogenetic proof was not available, the latter was probably involved in t(8;14). Restriction map analysis of one more case showed rearrangement on the pseudo-JH3 gene on one allele and t(14;18) on the other. Thus, in 11 of 19 cases, defective Ig H chain production could be explained by the inactivation of both Ig H chain genes due to translocation of one allele, in combination with deletions or defective rearrangements of the other allele. In contrast, in 28 of 30 Ig+ lymphomas, one functional Ig H chain allele was found, either in, or not in, combination with t(14;18). In two cases a single rearranged Ig H chain allele was found in combination with rearrangement of bcl-2. No comigration of the single Ig rearrangement with bcl-2, however, was found both by Southern blotting and PCR, suggesting a variant bcl-2 translocation, which leaves the Ig H chain allele functionally intact. |
| Starting Page | 613 |
| Ending Page | 624 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 2538543 |
| Volume Number | 169 |
| Journal | The Journal of experimental medicine |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/0a/c1/je1693613.PMC2189270.pdf |
| Alternate Webpage(s) | http://jem.rupress.org/content/jem/169/3/613.full.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acute lymphocytic leukemia Antibody Formation BCL2 gene Chromosomal translocation Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 8 DNA Sequence Rearrangement Karyotype determination procedure Leukemia, B-Cell Lymphoma MYC wt Allele Ovarian Follicle Polymerase Chain Reaction Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Pseudo brand of pseudoephedrine Southern blot assay (procedure) lymphoblast t(14;18) t(8;14) |
| Content Type | Text |
| Resource Type | Article |
