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Anti-inflammatory effect of pyroglutamyl-leucine on lipopolysaccharide-stimulated RAW 264.7 macrophages.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hirai, Shizuka Horii, Sho Matsuzaki, Yoshiaki Ono, Shin Ichi Shimmura, Yuki Sato, Kenji Egashira, Yukari |
| Copyright Year | 2014 |
| Abstract | AIMS Food-derived peptides have been reported to yield a variety of health promoting activities. Pyroglutamyl peptides are contained in the wheat gluten hydrolysate. In the present study, we investigated the effect of pyroglutamyl dipeptides on the lipopolysaccharide (LPS)-induced inflammation in macrophages. MAIN METHODS RAW 264.7 macrophages were treated with LPS and various concentrations of pyroglutamyl-leucine (pyroGlu-Leu), -valine (pyroGlu-Val), -methionine (pyroGlu-Met), and -phenylalanine (pyroGlu-Phe). Cell viability/proliferation and various inflammatory parameters were measured by the established methods including ELISA and western blotting. The binding of fluorescein isothiocyanate-labeled LPS to RAW 264.7 cells was also measured fluorescently. KEY FINDINGS All the tested dipeptides significantly inhibited the secretion of nitric oxide, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 from LPS-stimulated RAW 264.7 macrophages. Above all, pyroGlu-Leu inhibited the secretion of all these inflammatory mediators even at the lowest dose (200μg/ml). PyroGlu-Leu dose-dependently suppressed IκBα degradation and MAPK (JNK, ERK, and p38) phosphorylation in LPS-stimulated RAW 264.7 cells. On the other hand, it did not affect the binding of LPS to the cell surface. SIGNIFICANCE Our results indicated that pyroGlu-Leu inhibits LPS-induced inflammatory response via the blocking of NF-κB and MAPK pathways in RAW 264.7 macrophages. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.lfs.2014.08.017 |
| PubMed reference number | 25225121 |
| Journal | Medline |
| Volume Number | 117 |
| Issue Number | 1 |
| Alternate Webpage(s) | https://researchmap.jp/?action=cv_download_main&upload_id=87476 |
| Alternate Webpage(s) | https://doi.org/10.1016/j.lfs.2014.08.017 |
| Journal | Life sciences |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
