NDLI: Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

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Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides

Content Provider	Semantic Scholar
Author	Nordhoff, E. Kirpekar, Finn Karas, M. Cramer, Rainer Hahner, S. Hillenkamp, F. Kristiansen, Kurt Roepstroff, P. Lezius, A.
Copyright Year	1994
Abstract	UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
Starting Page	291
Ending Page	300
Page Count	10
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/22/13/10.1093_nar_22.13.2460/2/22-13-2460.pdf?Expires=1492746960&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=VxNnVbOPih2Ozc3GtDRQhIxfkysmb-KfAK-Df4Wzl6NxVd8buJpf~UI8-oqCR2jemAbuCc-s5hgOYXCbEd8uFL3KmZ7TAutqKEBk7J9vnqE9OxwY5Wf3Ku4Mx~PYgMh~aLaKqud7tp6aoz~UpA0EM8IrOZnQ8XVKQ15-DTriFp3UzA4gmZcXcpWECATYz4Hrd4bMOzrAnYlXAuSJAVx6r8ymaXxGEyarrInp6BSrvPgPFlvE7Dldeb1ab7Awds3~~Sa-kv3-8I4ITkWiDWOKldAlK-Y5cj-CgZPWuEKKJhCU1EhX-CMqs557aAaH98PlLfDsfjDIyVV8JcYvL~tiWg__
Alternate Webpage(s)	https://www.wikidata.org/entity/Q36735322
PubMed reference number	8041606v1
Alternate Webpage(s)	https://doi.org/10.1093/nar/22.13.2460
DOI	10.1093/nar/22.13.2460
Journal	Nucleic Acids Research
Volume Number	22
Issue Number	13
Language	English
Access Restriction	Open
Subject Keyword	3-hydroxypicolinic acid Cell Nucleus Fragmentation (computing) Ions Nucleic Acids Nucleotides Oligodeoxyribonucleotides RNA Reflectron Spectrometry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Succinic Acid
Content Type	Text
Resource Type	Article

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