NDLI: Collision-induced dissociation of singly charged peptide ions in a matrix-assisted laser desorption ionization ion trap mass spectrometer

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Collision-induced dissociation of singly charged peptide ions in a matrix-assisted laser desorption ionization ion trap mass spectrometer

Content Provider	Semantic Scholar
Author	Qin, Jun Chait, Brian T.
Copyright Year	1999
Abstract	We document the systematics of collision-induced dissociation of singly charged peptide ions in a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer. We show that singly charged peptide ions with m/z ratios extending to 3500 can be effectively fragmented using a newly devised excitation scheme, termed red shifted off-resonance large amplitude excitation (RSORLAE), and classify the dominant features of the resulting collision-induced dissociation spectra as follows. (1) Peptides ions with a m/zvalue of,1500 can be effectively fragmented independent of their amino acid composition. (2) Peptides ions with m/z , 3000 that contain lysine but not arginine can be extensively fragmented. (3) Arginine-containing peptide ions undergo highly preferential fragmentation at the C-termini of aspartic/glutamic acid residues. (4) Peptide ions containing a C-terminal lysine residue as well as one or more arginine residues readily rearrange to lose the terminal lysine. (5) Proline-containing peptide ions fragment preferentially at the N-termini of the proline residues. These systematics are helpful for the verification of peptide primary structures and for formulating efficient strategies for protein identification using tandem MALDI ion trap mass spectrometric data. (Int J Mass Spectrom 190/191 (1999) 313–320) © 1999 Elsevier Science B.V.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://lab.rockefeller.edu/chait/pdf/99/99_qin_int-j-mass.pdf
Language	English
Access Restriction	Open
Subject Keyword	Amino Acids Arginine Charge (electrical) Classification Diffuse Large B-Cell Lymphoma Excitation Fragmentation (computing) Glutamic Acid Ion Trap Ions Lysine Mass Spectrometers (device) Proline Resonance Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization collision
Content Type	Text
Resource Type	Article

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