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Charakterisierung eines transgenen Mausmodells mit spezifischer zytosolischer Expression des optischen Redox-Indikators roGFP1 in Neuronen
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kolbrink, Benedikt |
| Copyright Year | 2015 |
| Abstract | Cellular redox alterations and reactive oxygen species (ROS) contribute to biochemical pathways and cellular signaling, but do also play a critical role in the development of neurodegenerative as well as neuropathological diseases. Genetically-encoded optical redox sensors have nowadays been shown to be superior to conventional redox-dyes, especially by allowing for dynamic and quantitative redox-imaging. In this dissertation a new transgenic mouse model with cytosolic expression of the optical redox-indicator roGFP1 in neurons under the Thy1-promotor (C57BL/6-TG(Thy1.2-roGFP1c)) has been characterized thoroughly. The transgenic animals underwent different behavioral tests and a healthy general phenotype of the animals was verified by assessment of various relevant physiological parameters. Measurements in acute brain slices and formalin-fixed brain sections with a 2-photon-laser-scanning-microscope and conventional wide-field fluorescence-microscopy confirmed a strong expression of roGFP1 in the CA1-region as well as in the neocortex and brain-stem. Neuron specific expression of roGFP1 was verified by counter staining with fluorophore-linked anti-NeuN-antibodies. The baseline of roGFP1-fluorescence and alterations in oxidation-levels in response to oxidants and reductants or during anoxia have been measured dynamically in real-time with fluorescence-microscopy in the hippocampal formation and the neocortex. It can be concluded that the examined C57BL/6-TG(Thy1.2-roGFP1c) mouse-model expresses functional redox sensors at sufficient levels in large parts of the brain. Availability of these mice will be crucial to further understand the role of reactive oxygen species and associated redox changes in both the physiological control of cellular function as well as in neuropathological processes. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ediss.uni-goettingen.de/bitstream/handle/11858/00-1735-0000-0023-960C-9/Fassung%20f%EF%BF%BDr%20SUB.pdf;jsessionid=B1952A2BCEB346A14AA3BF43F6EA7DD0?sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
