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Changes in Human Mucosal y 6 T Cell Repertoire and Function Associated with the Disease Process in Inflammatory Bowel Disease
| Content Provider | Semantic Scholar |
|---|---|
| Author | McVay, Laila D. Li, Baiqing Biancaniello, Renée Creighton, Mary Anne Bachwich, Dale Lichtenstein, Abba G. Rombeau, John L. Carding, Simon R. |
| Copyright Year | 2007 |
| Abstract | Background: Although y6 T cells are a major component of the human intestinal mucosa, it is not clear what role they play in mucosal inimunity or if they are involved in the disease process of inflammatory bowel disease (IBD). Materials and Methods: Flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were used to identify quantitative and qualitative changes in the repertoire of -y8T cells present in surgical and/or biopsy samples of normal and inflamed colon from individual patients with ulcerative colitis (UC) or Crohn's disease (CD). Cytokine production and the ability to adhere to and interact with colonic fibroblasts were used to compare the functional properties of yS T cells isolated from the normal and diseased colonic mucosa. Results: Increased numbers of yS T cells localized in areas of inflammation and tissue injury were found in the majority of patients, irrespective of the type of IBD present. This expansion was attributable to an increase in V61+ cells expressing a V5I-(D63)-J&1-encoded T cell receptor and was seen in patients with severe disease as well as those with newly diagnosed or less severe forms of IBD. Among T cells present in the inflamed mucosa of patients with CD, y8T cells, particularly V81 + cells, were a major source of the proinflammatory cytokine interferon--y and could interact with colonic fibroblasts. Conclusions: Our results demonstrate that the chronic inflammatory immune response characteristic of IBD is associated with distinct changes in the number, distribution, composition, and function of mucosal 'yS T cells. Through the production of cytokines and physical interaction with other cells, y8 T cells can perform an immunoregulatory function and contribute to the pathophysiology of IBDs. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.molmed.org/content/1997/3_97/McVay.PDF |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Binding (Molecular Function) Colon classification Colon structure (body structure) Crohn Disease Disease gene identification Human–computer interaction Inflammatory Bowel Diseases Intestinal Diseases Intestinal Mucosa Intestines Ion beam deposition Irritable Bowel Syndrome Leukemia, B-Cell Mucous Membrane Patients RNA-Directed DNA Polymerase Reverse Transcriptase Polymerase Chain Reaction Soft Tissue Injuries UC Browser Ulcer Ulcerative Colitis cytokine |
| Content Type | Text |
| Resource Type | Article |
