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Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased in inflammatory bowel disease and colon cancer
| Content Provider | Semantic Scholar |
|---|---|
| Author | Prorok-Hamon, Maelle Friswell, Melissa K. Alswied, Abdullah M. Roberts, Carol L. Song, Fei Flanagan, Paul Kevin Knight, Paul G. Codling, Caroline E. Marchesi, Julian R. Winstanley, Craig Hall, Neil Rhodes, Jh. Campbell, Barry J. |
| Copyright Year | 2014 |
| Abstract | OBJECTIVE Colonic mucosa-associated Escherichia coli are increased in Crohn's disease (CD) and colorectal cancer (CRC). They variously haemagglutinate, invade epithelial cell lines, replicate within macrophages, translocate across M (microfold) cells and damage DNA. We investigated genes responsible for these effects and their co-association in colonic mucosal isolates. DESIGN A fosmid library yielding 968 clones was prepared in E coli EPI300-T1 using DNA from a haemagglutinating CRC isolate, and resulting haemagglutinating clones were 454-pyrosequenced. PCR screening was performed on 281 colonic E coli isolates from inflammatory bowel disease (IBD) (35 patients), CRC (21) and controls (24; sporadic polyps or irritable bowel syndrome). RESULTS 454-Pyrosequencing of fosmids from the haemagglutinating clones (n=8) identified the afimbrial adhesin afa-1 operon. Transfection of afa-1 into E coli K-12 predictably conferred diffuse adherence plus invasion of HEp-2 and I-407 epithelial cells, and upregulation of vascular endothelial growth factor. E coli expressing afaC were common in CRC (14/21, p=0.0009) and CD (9/14, p=0.005) but not ulcerative colitis (UC; 8/21) compared with controls (4/24). E coli expressing both afaC and lpfA (relevant to M-cell translocation) were common in CD (8/14, p=0.0019) and CRC (14/21, p=0.0001), but not UC (6/21) compared with controls (2/24). E coli expressing both afaC and pks (genotoxic) were common in CRC (11/21, p=0.0015) and UC (8/21, p=0.022), but not CD (4/14) compared with controls (2/24). All isolates expressed dsbA and htrA relevant to intra-macrophage replication, and 242/281 expressed fimH encoding type-1 fimbrial adhesin. CONCLUSIONS IBD and CRC commonly have colonic mucosal E coli that express genes that confer properties relevant to pathogenesis including M-cell translocation, angiogenesis and genotoxicity. |
| Starting Page | 761 |
| Ending Page | 770 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/early/2013/07/10/gutjnl-2013-304739.full.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp4.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp2.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp6.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp3.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp5.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/suppl/2013/07/10/gutjnl-2013-304739.DC1/gutjnl-2013-304739supp1.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/37/10/gutjnl-2013-304739.PMC3995253.pdf |
| Alternate Webpage(s) | http://gut.bmj.com/content/gutjnl/63/5/761.full.pdf |
| PubMed reference number | 23846483 |
| Alternate Webpage(s) | https://doi.org/10.1136/gutjnl-2013-304739 |
| DOI | 10.1136/gutjnl-2013-304739 |
| Journal | Gut |
| Volume Number | 63 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adhesins, Bacterial Colon Carcinoma Colon structure (body structure) Colorectal Carcinoma Crohn Disease Escherichia coli HTRA1 gene Intestinal Diseases Intestines Irritable Bowel Syndrome Mucous Membrane Operon Patients Ulcer Ulcerative Colitis polyps |
| Content Type | Text |
| Resource Type | Article |
