NDLI: Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations.

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Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations.

Content Provider	Semantic Scholar
Author	Takahashi-Fujii, Asako Ishino, Yoshio Shimada, Akiko
Copyright Year	1993
Abstract	We present a practical application of polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP) using a fluorescence-based image analyzer. Fluorescence-labeled primers were used to amplify target sequences. After nondenaturing polyacrylamide gel electrophoresis using a conventional sequencing gel apparatus, the glass plate was directly transferred from the gel box to the image analyzer. High-quality computer storage of the imaging data allowed the image intensity to be quantified over a wide range. Various data bases can be constructed on the basis of this imaging data. This system is suitable for use in clinical examination settings, and its application, to detect ras gene mutations in human pancreatic cancer specimens, is presented.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://genome.cshlp.org/content/2/4/323.full.pdf
PubMed reference number	8324506v1
Volume Number	2
Issue Number	4
Journal	PCR methods and applications
Language	English
Access Restriction	Open
Subject Keyword	Analyzer Device Component Analyzer, device Base Biopolymer Sequencing Fluorescence Gel Electrophoresis (lab technique) Pancreatic carcinoma Polyacrylamide Gel Electrophoresis Polymerase Chain Reaction SSCP Analysis Specimen Tracer polyacrylamide gels
Content Type	Text
Resource Type	Article

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