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Measles virus genotyping by nucleotide-specific multiplex PCR.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kremer, Jacques R. Fack, Fred Olinger, Christophe M. Mulders, Mick N. Muller, Claude P. |
| Copyright Year | 2004 |
| Abstract | A simple genotyping method based on multiplex PCR has been developed to discriminate between all active measles virus (MV) clades and genotypes (A, B3.1, B3.2, C2, D2-D9, G2-G3, and H1-H2). The sequencing reaction was replaced by six multiplex PCRs: one to identify the clade and five to identify the respective genotype. Primers were sensitive to clade- and genotype-specific nucleotides and generated fragments of type-specific sizes that were analyzed by conventional agarose gel electrophoresis. On the basis of all published MV sequences, positive and negative predictive values of 99.2% and 98.6% were calculated. Variability in the primer binding sites, which could potentially reduce sensitivity, was very limited among published sequences. As new genotypes are described, additional specific primers can be included in the multiplex PCR with relative ease. Although sequencing remains the "gold standard," the present method should facilitate MV genotyping especially in developing countries and will therefore contribute to enhanced MV control and elimination strategies as recommended by the World Health Organization. |
| Starting Page | 1378 |
| Ending Page | 1381 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jcm.asm.org/content/42/7/3017.full.pdf |
| PubMed reference number | 15243053v1 |
| Volume Number | 42 |
| Issue Number | 7 |
| Journal | Journal of clinical microbiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Agarose Gel Arthrogryposis Binding Sites Biopolymer Sequencing Electrophoresis, Agar Gel Excretory function Gel Electrophoresis (lab technique) Genotype determination Murine sarcoma viruses Nucleotides Reverse Transcriptase Polymerase Chain Reaction Scientific Publication |
| Content Type | Text |
| Resource Type | Article |
