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Assembly of hepatitis delta virus: particle characterization, including the ability to infect primary human hepatocytes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | He, Yiping Meier, Anja Chang, Jinhong Chen, Rongji Jarnik, Michal Nicolas, Emmanuelle Bruss, Volker |
| Copyright Year | 2007 |
| Abstract | Efficient assembly of hepatitis delta virus (HDV) was achieved by cotransfection of Huh7 cells with two plasmids: one to provide expression of the large, middle, and small envelope proteins of hepatitis B virus (HBV), the natural helper of HDV, and another to initiate replication of the HDV RNA genome. HDV released into the media was assayed for HDV RNA and HBV envelope proteins and characterized by rate-zonal sedimentation, immunoaffinity purification, electron microscopy, and the ability to infect primary human hepatocytes. Among the novel findings were that (i) immunostaining for delta antigen 6 days after infection with 300 genome equivalents (GE) per cell showed only 1% of cells as infected, but this was increased to 16% when 5% polyethylene glycol was present during infection; (ii) uninfected cells did not differ from infected cells in terms of albumin accumulation or the presence of E-cadherin at cell junctions; and (iii) sensitive quantitative real-time PCR assays detected HDV replication even when the multiplicity of infection was 0.2 GE/cell. In the future, this HDV assembly and infection system can be further developed to better understand the mechanisms shared by HBV and HDV for attachment and entry into host cells. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://jvi.asm.org/content/81/7/3608.full.pdf |
| PubMed reference number | 17229685v1 |
| Volume Number | 81 |
| Issue Number | 7 |
| Journal | Journal of virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cadherins Equivalent Weight Hepatitis B Surface Antigen Vaccine Hepatitis B Virus Hepatitis D Infection Hepatitis Delta Virus Hepatitis delta Antigens Herpesvirus 1, Cercopithecine Intercellular Junctions Polyethylene Glycols RNA entry into host multiplicity |
| Content Type | Text |
| Resource Type | Article |
