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Establecimiento de un protocolo para la obtención de callos in vitro a partir de cotiledones de chocho andino (LUPINUS MUTABILIS)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Urbina, Villacis Alejandra, Paula |
| Copyright Year | 2017 |
| Abstract | Lupinus mutabilis, also known as chocho, is a legume that is cultivated in the Andean zone of South America. In Ecuador, chocho is cultivated in the region known as Sierra, mostly in the provinces of Imbabura, Chimborazo and Cotopaxi. The bean is known and consumed mainly due to its high protein content that represents approximately 40 percent of its composition. Chocho is a plant that is cultivated out in the open, for which it requires various stages for its production. In the main stages, there is a great demand for resources like water and energy. Also, it is common that crops suffer from plagues and illnesses that causes loss in the final product. In vitro culture of chocho is not a much studied area due to the high percentage of contamination and release of oxidizing compounds. The object of this investigation was to establish a protocol for a callus culture using chocho cotyledons. First a disinfection protocol was established for seeds. 6 treatments where tested, results showed that treatment number 6 was the best for producing viable explants. Treatment 6 consisted of submerging the seed in ethanol 70 percent and a solution of sodium hypochlorite 7 percent. A culture medium for callus formation was also formulated. It contained MS (Murashige and Skoog) macro and microelements 6-BAP (0.5 mg/L), ANA (1.5 mg/L), and an antioxidant, in this case active carbon (2 g/L). Through the disinfection protocol it was possible to obtain 38.88 percent of viable explants (free of contamination and necrosis). When these explants were transferred to the callus medium, there exists callus formation with a maximum weight of 219.5 mg. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dspace.udla.edu.ec/bitstream/33000/8192/1/UDLA-EC-TIB-2017-45.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
