NDLI: Translational repression in eukaryotes: partial purification and characterization of a repressor of ferritin mRNA translation.

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Translational repression in eukaryotes: partial purification and characterization of a repressor of ferritin mRNA translation.

Content Provider	Semantic Scholar
Author	Walden, William E. Daniels-Mcqueen, S. M. Brown, Patrick H. Gaffield, Livia Russell, Douglas A. Bielser, Daniel Bailey, Lynne C. Thach, R. E.
Copyright Year	1988
Abstract	Mouse and rabbit ferritin mRNAs translate very poorly in rabbit reticulocyte lysates relative to most other mRNAs. This translational deficiency is not seen in wheat germ lysates, suggesting the presence of an inhibitor in reticulocyte lysate that is specific for ferritin mRNA. A specific repressor of ferritin mRNA translation has been partially purified from rabbit reticulocytes by differential ultracentrifugation, ammonium sulfate fractionation, and chromatography on phosphocellulose, DEAE-cellulose, and Sephacryl S-300. The elution profile from the latter suggests an aggregate molecular mass of approximately 180 kDa for the repressor. The inhibitory activity of this repressor against native ferritin mRNA can be relieved by adding in vitro transcripts of ferritin light-chain RNAs that contain the first 92 nucleotides of the 5' untranslated region. No other sequences appear to be necessary for this effect.
Starting Page	39
Ending Page	45
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/85/24/9503.full.pdf
PubMed reference number	3200835v1
Volume Number	85
Issue Number	24
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	2-diethylaminoethanol 5' Untranslated Regions Ammonium Sulfate Cellulose DEAE-Cellulose Ferritin Genetic Translation Process Lysate Molecular Mass Nucleotides Repression, Psychology Reticulocytes Transcript Transcription Repressor/Corepressor Translational Repression Ultracentrifugation
Content Type	Text
Resource Type	Article

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