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Role of the transcription activator Ste12p as a repressor of PRY3 expression.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bickel, Kellie S. Morris, D. R. |
| Copyright Year | 2006 |
| Abstract | Mating pheromone represses synthesis of full-length PRY3 mRNA, and a new transcript appears simultaneously with its 5' terminus 452 nucleotides inside the open reading frame (ORF). Synthesis of this shorter transcript results from activation of a promoter within the PRY3 locus, and its production is concomitant with the rapid disappearance of the full-length transcript. Evidence is consistent with the pheromone-induced transcription factor Ste12p binding two pheromone response elements within the PRY3 promoter, directly impeding transcription of the full-length mRNA while simultaneously inducing initiation of the short transcript. This process depends on a TATA box within the PRY3 ORF. Expression of full-length PRY3 inhibited mating, while no disadvantage was detectable for cells unable to make the short transcript. Therefore, Ste12p is utilized as a repressor of full-length PRY3 transcription, ensuring efficient mating. There is no evidence that production of the short PRY3 transcript is anything more than an adventitious by-product of this mechanism. It is possible that cryptic binding sites for transcriptional activators may occur frequently within genomes and have the potential of evolving for rapid, gene-specific repression by mechanisms analogous to PRY3. PRY3 regulation provides a model for the coordination of both inductive and repressive activities within a regulatory network. |
| Starting Page | 1 |
| Ending Page | 5 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://mcb.asm.org/content/26/21/7901.full.pdf |
| PubMed reference number | 16940175v1 |
| Volume Number | 26 |
| Issue Number | 21 |
| Journal | Molecular and cellular biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Binding Sites Genome Nucleotides Open Reading Frames Reading Frames (Nucleotide Sequence) Repression, Psychology Response Elements TRANSCRIPTION FACTOR Transcript Transcription Coactivator Transcription Initiation Transcription Repressor/Corepressor Transcription, Genetic response to pheromone |
| Content Type | Text |
| Resource Type | Article |
