DNA polymerase of bacteriophage T4 is an autogenous translational repressor.

Content Provider	Semantic Scholar
Author	Andrake, Mark D. Guild, N. Hsu, Tsungda Gold, Larry Tuerk, C. Karam, Jim D.
Copyright Year	1988
Abstract	In bacteriophage T4 the protein product of gene 43 (gp43) is a multifunctional DNA polymerase that is essential for replication of the phage genome. The protein harbors DNA-binding, deoxyribonucleotide-binding, DNA-synthesizing (polymerase) and 3'-exonucleolytic (editing) activities as well as a capacity to interact with several other T4-induced replication enzymes. In addition, the T4 gp43 is a repressor of its own synthesis in vivo. We show here that this protein is an autogenous repressor of translation, and we have localized its RNA-binding sequence (translational operator) to the translation initiation domain of gene 43 mRNA. This mechanism for regulation of T4 DNA polymerase expression underscores the ubiquity of translational repression in the control of T4 DNA replication. Many T4 DNA polymerase accessory proteins and nucleotide biosynthesis enzymes are regulated by the phage-induced translational repressor regA, while the T4 single-stranded DNA-binding protein (T4 gp32) is, like gp43, autogenously regulated at the translational level.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/85/21/7942.full.pdf
PubMed reference number	3054876v1
Volume Number	85
Issue Number	21
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Anabolism Bacteriophage T4 Bacteriophages DNA Breaks, Single-Stranded DNA Replication Deoxyribonucleotides Genetic Translation Process Nucleotide Biosynthesis Nucleotides RNA Repression, Psychology Transcription Initiation Transcription Repressor/Corepressor Translation Initiation Translational Repression negative regulation of DNA-directed DNA polymerase activity
Content Type	Text
Resource Type	Article