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The Cdk5-p35 kinase associates with the Golgi apparatus and regulates membrane traffic.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Paglini, Gabriela Peris, Leticia Díez-Guerra, Javier Quiroga, Santiago Cáceres, Alfredo |
| Copyright Year | 2001 |
| Abstract | We show here that an active Cdk5-p35 kinase is present in Golgi membranes, where it associates with a detergent-insoluble fraction containing actin. In addition, Cdk5-p35-dependent phosphorylation of alpha-PAK immunoreactive protein species was detected in Golgi membranes, as well as an interaction with the small GTPase, Cdc42. Moreover, antisense oligonucleotide suppression of Cdk5 or p35 in young cultured neurons, as well as inhibition of Cdk5 activity with olomoucine, blocks the formation of membrane vesicles from the Golgi apparatus. Taken together, these results show a novel subcellular localization of this kinase and suggest a role for Cdk5-p35 in membrane traffic during neuronal process outgrowth. |
| Starting Page | 29 |
| Ending Page | 40 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://embor.embopress.org/content/embor/3/3/286.full.pdf |
| PubMed reference number | 11743029v1 |
| Volume Number | 2 |
| Issue Number | 12 |
| Journal | EMBO reports |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Detergents Glycoprotein Hormones, alpha Subunit Golgi Apparatus Guanosine Triphosphate Phosphohydrolases Membrane Protein Traffic Tissue membrane Vesicle (morphologic abnormality) antisense therapy olomoucine positive regulation of ER to Golgi vesicle-mediated transport |
| Content Type | Text |
| Resource Type | Article |
