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Exomer: a coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, C. Y. Hamamoto, Susan T. Orci, Lelio Schekman, Randy |
| Copyright Year | 2006 |
| Abstract | A yeast plasma membrane protein, Chs3p, transits to the mother-bud neck from a reservoir comprising the trans-Golgi network (TGN) and endosomal system. Two TGN/endosomal peripheral proteins, Chs5p and Chs6p, and three Chs6p paralogues form a complex that is required for the TGN to cell surface transport of Chs3p. The role of these peripheral proteins has not been clear, and we now provide evidence that they create a coat complex required for the capture of membrane proteins en route to the cell surface. Sec7p, a Golgi protein required for general membrane traffic and functioning as a nucleotide exchange factor for the guanosine triphosphate (GTP)-binding protein Arf1p, is required to recruit Chs5p to the TGN surface in vivo. Recombinant forms of Chs5p, Chs6p, and the Chs6p paralogues expressed in baculovirus form a complex of approximately 1 MD that binds synthetic liposomes in a reaction requiring acidic phospholipids, Arf1p, and the nonhydrolyzable GTPgammaS. The complex remains bound to liposomes centrifuged on a sucrose density gradient. Thin section electron microscopy reveals a spiky coat structure on liposomes incubated with the full complex, Arf1p, and GTPgammaS. We termed the novel coat exomer for its role in exocytosis from the TGN to the cell surface. Unlike other coats (e.g., coat protein complex I, II, and clathrin/adaptor protein complex), the exomer does not form buds or vesicles on liposomes. |
| Starting Page | 973 |
| Ending Page | 983 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 17000877 |
| Volume Number | 174 |
| Journal | The Journal of cell biology |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/63/bd/jcb1740973.PMC2064389.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acids Adaptor Proteins, Signal Transducing Bud - CHV concept Clathrin Electron Microscopy Exocytosis Golgi Apparatus Gradient Guanine Nucleotide Exchange Factors Guanosine Triphosphate Liposomes Membrane Protein Traffic Membrane Proteins Phospholipids Plasma membrane Reservoir Device Component Sucrose Thin Layer Chromatography Vesicle (morphologic abnormality) incubated positive regulation of ER to Golgi vesicle-mediated transport |
| Content Type | Text |
| Resource Type | Article |
