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Stable isotope labeling by amino acids in cell culture (SILAC).

Content Provider	Semantic Scholar
Author	Gruhler, Siegfried Kratchmarova, Irina
Copyright Year	2008
Abstract	Quantitative proteomics has become a pivotal tool that has been applied to the investigation of many different biological processes such diverse as the detection of biomarkers in tissue samples, the regulation of cell signaling, and the characterization of protein interactions. Stable isotope labeling techniques have facilitated the precise quantitation of changes in protein abundance by mass spectrometry. Among different choices, Stable Isotope Labeling by Amino acids in Cell culture (SILAC) is an easy and reliable method for unbiased comparative proteomic experiments, which has been employed to study post-translational modifications such as protein phosphorylation and methylation, to characterize signaling pathways and to determine specific protein interactions. Here we describe detailed procedures for SILAC experiments in mammalian and yeast cells.
Starting Page	3991
Ending Page	4024
Page Count	34
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.isotope.com/userfiles/files/assetLibrary/PRT_PANDEY.pdf
Alternate Webpage(s)	http://cseweb.ucsd.edu/classes/fa05/cse182/slides/silac.pdf
Alternate Webpage(s)	http://www.mcponline.org/content/1/5/376.full.pdf
Alternate Webpage(s)	http://cseweb.ucsd.edu/classes/fa09/cse182/slides/silac.pdf
Alternate Webpage(s)	http://cseweb.ucsd.edu/classes/sp10/cse283/notes/silac.pdf
Alternate Webpage(s)	http://mcponline.highwire.org/content/1/5/376.full.pdf
Alternate Webpage(s)	http://www.phas.ubc.ca/~chansen/Papers%20Covered/mass%20spec/Ong%20J%20Prot%20Res%202003.pdf
PubMed reference number	18369856v1
Alternate Webpage(s)	https://doi.org/10.1007/978-1-60327-064-9_9
DOI	10.1007/978-1-60327-064-9_9
Journal	Methods in molecular biology
Volume Number	424
Language	English
Access Restriction	Open
Subject Keyword	Amino Acids Biological Factors Biological Markers Cell Culture Techniques Choice Behavior Genetic Translation Process Isotope Labeling Isotopes Mammals Post-Translational Protein Processing Protein phosphorylation Proteomics Quantitation Spectrometry Stable angina
Content Type	Text
Resource Type	Article

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