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Stable isotope labeling in mammals (SILAM).
| Content Provider | Semantic Scholar |
|---|---|
| Author | McClatchy, Daniel B. Yates, John R. |
| Copyright Year | 2014 |
| Abstract | Analysis of animal models of disease is essential to the understanding of human disease and the identification of potential targets for clinical drugs. Global analysis of proteins by mass spectrometry is an important tool for these studies. Stable isotope labeling in mammals (SILAM) was developed to quantitate the proteomes of rodents using mass spectrometry. The crux of SILAM analysis is the complete labeling of all proteins in a rodent with heavy nitrogen ((15)N). These (15)N tissues are then employed as an internal standard for quantitative proteomics analysis using a high-resolution and mass-accuracy mass spectrometer. |
| Starting Page | 92 |
| Ending Page | 110 |
| Page Count | 19 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ckisotopes.com/wp-content/uploads/2015/04/Stable-Isotope-Labeling-in-Mammals-SILAM-John-Yates.pdf |
| PubMed reference number | 24791985v1 |
| Alternate Webpage(s) | https://doi.org/10.1007/978-1-4939-0685-7_8 |
| DOI | 10.1007/978-1-4939-0685-7_8 |
| Journal | Methods in molecular biology |
| Volume Number | 1156 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Animal Model Body tissue Isotope Labeling Isotopes Mammals Mass Spectrometers (device) Mass Spectrometry Proteomics Rodent Stable angina |
| Content Type | Text |
| Resource Type | Article |
