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Proteomics study reveals cross-talk between Rho guanidine nucleotide dissociation inhibitor 1 post-translational modifications in epidermal growth factor stimulated fibroblasts.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Guerrera, Ida Chiara Keep, Nicholas H. Godovac-Zimmermann, Jasminka |
| Copyright Year | 2007 |
| Abstract | Following stimulation of NRK49F rat kidney fibroblast cells with epidermal growth factor, possible preemptive cross-talk between arginine methylation and serine and tyrosine phosphorylation was observed for Rho guanidine nucleotide dissociation inhibitor 1 (RhoGDI-1). Five dimethylation sites (Lys50, Lys52, Arg111, Arg152, Arg180) and two new phosphorylation sites (Tyr144, Ser148) were identified for RhoGDI-1. All presently known phosphorylation sites for RhoGDI-1 lie within the 10 residues immediately prior to the 3 sites for arginine dimethylation, and these dimethylation/phosphorylation modules may constitute functional switches. Consideration of structural data and other literature for RhoGDI-1 suggests that methylation and phosphorylation cooperatively affect formation of complexes with different Rho/Rac family proteins and that methylation may be crucial in partitioning of RhoGDI-1 between different functional roles. On the basis of results presented here, it can be implied that unidentified arginine methyltransferases may exist and that arginine methylation may have a greater role in cellular signaling processes than is currently recognized. The combined use of SILAC labeling of arginine (SILAC = stable isotope labeling by amino acids in cell culture), immobilized metal affinity chromatography based phosphoprotein enrichment, and mass spectrometry is clearly a useful method for this investigation. |
| Starting Page | S415 |
| Ending Page | S415 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.proteomics.necker.fr/pdfs/rhogdi-icg.pdf |
| PubMed reference number | 17506542v1 |
| Volume Number | 6 |
| Issue Number | 7 |
| Journal | Journal of proteome research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Arginine Cell Culture Techniques Chromatography, Affinity Cross Reactions Cross-Talk Genetic Translation Process Growth Factor Guanidine Isotope Labeling Isotopes Methylation Nucleotides Phosphoproteins Post-Translational Protein Processing Proteomics Spectrometry Switch Device Component Tyrosine Phosphorylation negative regulation of pyrimidine nucleotide biosynthetic process rho Guanine Nucleotide Dissociation Inhibitor beta rho-Associated Kinases stable isotope |
| Content Type | Text |
| Resource Type | Article |
