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Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jäck, Heinz Mcdowell, Mindy Steinberg, Charles M. Wabl, Matthias R. |
| Copyright Year | 1988 |
| Abstract | In the mouse pre-B-cell line 18-81, cells can switch production in vitro from immunoglobulin mu chain to gamma 2b chain. The gene encoding the gamma 2b chain is created by a rearrangement of the mu gene. This rearrangement always takes place within a homolog. In cells with a gamma 2b gene, most of the time the gene segment encoding the constant region of the mu chain is deleted, but often the rearrangement leads to cells that produce no immunoglobulin, and all DNA sequences are retained. The latter result is due to an inversion. Inversions exclude the unequal sister chromatid exchange model of the heavy-chain class switch. Looping out is an intermediate step in the process of generating an inversion. Our findings demonstrate that the switch rearrangement occurs by looping out and deletion. |
| Starting Page | 155 |
| Ending Page | 166 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/85/5/1581.full.pdf |
| PubMed reference number | 2830623v1 |
| Volume Number | 85 |
| Issue Number | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | B-Lymphocytes Chromatids Chromosome inversion DNA Sequence Rearrangement Deletion Mutation Immunoglobulin mu-Chains Pre-B Lymphocytes Sister Chromatid Exchange |
| Content Type | Text |
| Resource Type | Article |
