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Double recombination of a single immunoglobulin kappa-chain allele: implications for the mechanism of rearrangement.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Feddersen, Rodney M. Ness, Brian G. Van |
| Copyright Year | 1985 |
| Abstract | DNA fragments containing immunoglobulin kappa-chain sequences from two different plasmacytomas (PC 3609 and PC 7043) were found by blot-hybridization studies to be dissociated from germ-line sequences on both the 3' and 5' ends. These fragments were cloned, sequenced, and found to contain the structural features of a product of two recombination events. Each contained a variable (V kappa) gene segment recombined with a joining (J kappa) gene segment followed by the characteristic kappa light chain V-J reciprocal structure, a 5' J kappa flanking sequence joined to a 3' V kappa flanking sequence. These segments of DNA represent double recombination products (DRPs) of the same kappa-chain allele. The DRP from PC 3609 contains a normal V-J1 recombination, while the DRP from PC 7043 contains an aberrant V-J2 recombination, resulting in a frameshift. The reciprocal structure in the PC 3609 DRP is the result of a V-J2 recombination; the reciprocal structure in the DRP of PC 7043 is the result of a V-J3 recombination and appears to have been derived directly from the productive kappa-chain gene recombination in that plasmacytoma. These products demonstrate the capacity of a single kappa light chain immunoglobulin allele to undergo multiple V-J recombinations. Furthermore, the presence of a V-J recombination and its reciprocal product in the same cell is inconsistent with a segregating mechanism, such as sister chromatid exchange, but is consistent with an inversion mechanism. |
| Starting Page | 2628 |
| Ending Page | 2635 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/82/14/4793.full.pdf |
| PubMed reference number | 2991895v1 |
| Volume Number | 82 |
| Issue Number | 14 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Chromatids Clone Cells Contain (action) DNA Breaks, Double-Stranded DNA Sequence Rearrangement Flank (surface region) Frameshift Mutation function Immunoglobulin kappa-Chains Nucleic Acid Hybridization Plasmacytoma Recombination, Genetic Sister Chromatid Exchange |
| Content Type | Text |
| Resource Type | Article |
