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Automated Multi-sample Solid-phase Dna Extraction on a Centrifugal Polyethylene Terephthalate ( Pe ) Microdevice
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jackson, Kimberly R. Borba, Juliane Cristina Meija, Maximo Garner, Gavin Carrilho, Emanuel Haverstick, D. M. Landers, James P. |
| Copyright Year | 2015 |
| Abstract | Dynamic solid-phase extraction (dSPE) has been explored in many microfluidic formats[1-4], however, most require complex fabrication, instrumentation with high-speed capabilities, are not amenable to post-process integration, and/or do not demonstrate multiplexed amplification required for fields, such as forensic DNA analysis. Previously, we presented initial efforts on a poly(ethylene terephthalate) (Pe) microfluidic device capable of producing PCR-ready DNA from 0.6 μL whole blood[5]. We now present an improved and automated Pe DNA dSPE device system capable of multiple extractions on an inexpensive low-speed rotational platform, yielding PCR-ready DNA for obtaining multiplexed PCR comparable to a commercial benchtop extraction. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rsc.org/images/LOC/2015/PDFs/Papers/1089_M.262d.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
