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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jackson, K. R. Borba, J. C. Meija, M. Mills, D. L. Haverstick, D. M. Olson, K. E. Aranda, R. Garner, G. T. Carrilho, E. Landers, J. P. |
| Description | Country affiliation: United States Author Affiliation: Jackson KR ( Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.); Borba JC ( Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos-SP, Brazil.); Meija M ( Department of Mechanical and Aerospace Engineering, University of Virginia, Charlottesville, VA 22904, USA.); Mills DL ( TeGrex Technologies, Sperryville, VA 22740, USA.); Haverstick DM ( Department of Pathology, University of Virginia, Charlottesville, VA 22904, USA.); Olson KE ( Office of the Chief Scientist, Defense Forensic Science Center, N 31st Street, Atlanta, GA 30297, USA.); Aranda R ( Office of the Chief Scientist, Defense Forensic Science Center, N 31st Street, Atlanta, GA 30297, USA.); Garner GT ( Department of Mechanical and Aerospace Engineering, University of Virginia, Charlottesville, VA 22904, USA.); Carrilho E ( Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos-SP, Brazil.); Landers JP ( Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA) |
| Abstract | We report the development of a disposable polyester toner centrifugal device for semi-automated, dynamic solid phase DNA extraction (dSPE) from whole blood samples. The integration of a novel adhesive and hydrophobic valving with a simple and low cost microfabrication method allowed for sequential addition of reagents without the need for external equipment for fluid flow control. The spin-dSPE method yielded an average extraction efficiency of â¼45% from 0.6 µL of whole blood. The device performed single sample extractions or accommodate up to four samples for simultaneous DNA extraction, with PCR-readiness DNA confirmed by effective amplification of a ß-globin gene. The purity of the DNA was challenged by a multiplex amplification with 16 targeted amplification sites. Successful multiplexed amplification could routinely be obtained using the purified DNA collected post an on-chip extraction, with the results comparable to those obtained with commercial DNA extraction methods. This proof-of-principle work represents a significant step towards a fully-automated low cost DNA extraction device. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Journal | Analytica Chimica Acta |
| Volume Number | 937 |
| e-ISSN | 18734324 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-09-21 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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