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Heterologously Expressed GLT-1 Associates in 200-nm Protein-Lipid Islands
| Content Provider | Semantic Scholar |
|---|---|
| Author | Raunser, Stefan Haase, Winfried Franke, Cornelia Eckert, Gunter P. Müller, Walter E. Kühlbrandt, Werner |
| Copyright Year | 2006 |
| Abstract | The glutamate transporter GLT-1 from Rattus norvegicus was expressed at high level in baby hamster kidney (BHK-21) cells by the Semliki Forest Virus expression system. We examined the expressed GLT-1 in the plasma membrane and found that the transporter accumulates in detergent-insoluble lipid-protein assemblies. Freeze-fracture, immunogold labeling, and electron microscopy revealed that GLT-1 forms ;200-nm protein-rich islands in the plasma membrane. Cholesterol depletion in living cells resulted in a dispersion of the GLT-1 islands, indicating that they are the result of lipid-protein rather than protein-protein interactions. Disruption of GLT-1 islands and dispersion of GLT-1 goes along with a reduction of the glutamate transport activity. Our direct visualization of lipid-protein islands in the plasma membrane of tissue culture cells suggests that the reported clustering of glutamate transporters and their cholesterol-dependent transport activity in cells is likewise connected to their association with cholesterol-rich microdomains in the plasma membrane. |
| File Format | PDF HTM / HTML |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cholesterol Cluster analysis Depletion region Detergents Electron Microscopy Glutamate Transporter Glutamic Acid High-level programming language Imagery Membrane Transport Proteins Plasma Active Plasma membrane Renal Tissue SLC1A2 gene SLC1A2 wt Allele protein protein interaction tissue culture |
| Content Type | Text |
| Resource Type | Article |
