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Neuronal glutamate transporter EAAT4 is expressed in astrocytes.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hu, Wenhui Walters, Winston M. Xia, Xiao-Mei Karmally, Shaffiat A. Bethea, John R. |
| Copyright Year | 2003 |
| Abstract | High-affinity excitatory amino acid transporters (EAATs) are essential to terminate glutamatergic neurotransmission and to prevent excitotoxicity. To date, five distinct EAATs have been cloned from animal and human tissues: GLAST (EAAT1), GLT-1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. EAAT1 and EAAT2 are commonly known as glial glutamate transporters, whereas EAAT3, EAAT4, and EAAT5 are neuronal. EAAT4 is largely expressed in cerebellar Purkinje cells. In this study, using immunohistochemistry and Western blotting, we found that EAAT4-like immunoreactivity (ir) is enriched in the spinal cord and forebrain. Double-labeled fluorescent immunostaining and confocal image analysis indicated that EAAT4-like ir colocalizes with an astrocytic marker, glial fibrillary acidic protein (GFAP). The astrocytic localization of EAAT4 was further confirmed in astrocyte cultures by double-labeled fluorescent immunocytochemistry and Western blotting. Reverse transcriptase-polymerase chain reaction analysis demonstrated mRNA expression of EAAT4 in astrocyte cultures. Sequencing confirmed the specificity of the amplified fragment. These results demonstrate that EAAT4 is expressed in astrocytes. This astrocytic localization of neuronal EAAT4 may reveal a new function of EAAT4 in the central nervous system. |
| Starting Page | 13 |
| Ending Page | 25 |
| Page Count | 13 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.people.vcu.edu/~whu/Publications.files/HuWH'sPub/EAA4-Glia.pdf |
| Alternate Webpage(s) | http://www.slas.ac.cn/upload/20071123-a5.pdf |
| Alternate Webpage(s) | http://web.slas.ac.cn/upload/20071123-a5.pdf |
| PubMed reference number | 12951653v1 |
| Volume Number | 44 |
| Issue Number | 1 |
| Journal | Glia |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acid Transporter Astrocytes CNS disorder Cerebellum Clone Cells Excitatory Amino Acids Glial Fibrillary Acidic Protein Glutamate Transporter Glutamic Acid Immunocytochemistry Membrane Transport Proteins Neuroglia Polymerase Chain Reaction Prosencephalon SLC1A1 gene SLC1A2 wt Allele SLC1A3 gene SLC1A6 gene SLC1A7 gene Spinal Cord Synaptic Transmission Thioctic Acid Tracer Western Blotting human tissue immunoreactivity |
| Content Type | Text |
| Resource Type | Article |
