NDLI: The action of phosphatidylinositol-specific phospholipases C on membranes.

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The action of phosphatidylinositol-specific phospholipases C on membranes.

Content Provider	Semantic Scholar
Author	Low, Martin G. Finean, J. B.
Copyright Year	1976
Abstract	A phospholipase C prepared from lymphocytes readily hydrolysed pure phosphatidyl-inositol but was relatively ineffective against phosphatidylinositol in erythrocyte "ghosts" and rat liver microsomal fraction and also against sonicated lipid extracts from these membranes. In contrast, a phospholipase C prepared from Staphylcoccus aureus readily hydrolysed phosphatidylinositol in sonicated lipid extracts but had only low activity against purified phosphatidylinositol. Unlike the enzyme from lymphocytes, the S. aureus phospholipase C did not require Ca2+ for its activity and was inhibited by cations. The previously reported specificity of this enzyme was confirmed by our observation of hydrolysis of approx. 75% of the phosphatidylinositol in ox, sheep and cat erythrocyte "ghosts" together with no detectable effect on the major erythrocyte membrane phospholipids. The phosphatidylinositol of rat liver microsomal fraction was hydrolysed only to a maximum of 15%. Some preliminary experiments showed that approx. 60% of the phosphatidylinositol of ox or sheep erythrocytes could be hydrolysed without causing substantial haemolysis.
Starting Page	77
Ending Page	81
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.biochemj.org/content/ppbiochemj/154/1/203.full.pdf
PubMed reference number	1275908v1
Volume Number	154
Issue Number	1
Journal	The Biochemical journal
Language	English
Access Restriction	Open
Subject Keyword	CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase Cations Erythrocyte Membrane Erythrocytes Hemolysis (disorder) Inositol Phosphatidylinositols Phospholipase C Phospholipids Tissue membrane Type C Phospholipases
Content Type	Text
Resource Type	Article

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