Loading...
Please wait, while we are loading the content...
Similar Documents
Evaluierung der biologischen Sicherheit von Xenotransplantaten: Untersuchungen zur Übertragung von porzinen endogenen Retroviren in vitro und in vivo
| Content Provider | Semantic Scholar |
|---|---|
| Author | Irgang, Markus |
| Copyright Year | 2004 |
| Abstract | Objective: Porcine endogenous retroviruses (PERVs) are integrated in the porcine genome and are able to infect human cells in vitro. Therefore, PERVs are one of the possible pathogens which poses a risk for xenotransplantations. In this study three significant methods were used to evaluate the infectious risk of PERV: The release of PERV particles from porcine cells and tissues, the formation of an in vivo infection model and a retrospective screening of patients. Methods: Islet cells from german landrace pigs were co-cultivated with human cells in vitro and were transplanted in BALB/c mice in vivo. Serial passaging experiments were performed with nonhuman primate cells. Murine cells were incubated in vitro and SCID mice in vivo with PERV. Sera of patients who were treated ex vivo with porcine liver cells and who had received islet cells were investigated for antibodies against PERV. Results: No virus release were observed in german landrace islet cells, thus they were neither able to infect human cells nor BALB/c mice. The used nonhuman primate cells released low replicating PERVs. None of the murine cells could be infected by PERV and no provirus integration was observed in different SCID mice organs. PERV-specific antibodies were found in none of the investigated patients. Conclusion: German landrace pigs could be used as a source for breeding safe genetically modified pigs suitable for xenotransplantation. Since there were no detectable PERV infection of different murine cells and SCID mice, it have to be supposed, that previously reported PERV transmissions to SCID mice might be due to microchimerism or to pseudotyping of murine endogenous retroviruses. Our results were confirmed by the fact, that the receptor for PERV-A is not expressed on murine cells and that these cells could not be infected in vitro. The absence of a PERV transmission in the investigated patients, correspond to the results obtained from approximately twohundred treated patients worldwide. |
| File Format | PDF HTM / HTML |
| DOI | 10.25646/5319 |
| Alternate Webpage(s) | https://edoc.rki.de/bitstream/handle/176904/5394/irgang.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | http://edoc.hu-berlin.de/dissertationen/irgang-markus-2005-03-23/PDF/Irgang.pdf |
| Alternate Webpage(s) | https://edoc.hu-berlin.de/bitstream/handle/18452/15957/Irgang.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://doi.org/10.25646/5319 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
