Loading...
Please wait, while we are loading the content...
Similar Documents
Human CD8+ TCR-αβ+ and TCR-γδ+ cells modulate autologous autoreactive neuroantigen-specific CD4+ T-cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Correale, Jorge Rojany, Micha Weiner, Leslie P. |
| Copyright Year | 1997 |
| Abstract | Ž . To investigate the regulatory interactions among autologous T-cells during the course of multiple sclerosis MS , proteolipid protein q Ž . peptide-specific CD4 T-cell clones TCCs were irradiated and used as immunogens to stimulate purified populations of autologous CD8 TCR-ab and TCR-gd T-cells isolated from the peripheral blood of MS patients, patients with other non-inflammatory neurological diseases, and healthy blood donors. The resulting blasts were expanded in the presence of hIL-2 and then cloned by limiting dilution. Two different groups of CD8 TCCs were revealed. A first group of CD8 TCCs recognized autologous CD4 T-cells based in Ž . q q their TCRVb structures anti-idiotypic responsiveness . A second group of CD8 TCCs recognized Ag activated autologous CD4 TCCs Ž . irrespective of their Ag specificity or TCRVb expression anti-ergotypic responsiveness . Both groups showed MHC class I restricted cytotoxicity against CD4 T-cells and were able to secrete IFN-g , TNFarb and TGF-b. TCR-gd TCCs isolated in response to stimulation with autologous peptide-specific CD4 TCCs showed only anti-ergotypic cytotoxicity, which was not inhibited by anti-MHC class Ia monoclonal antibodies. Moreover, they were able to secrete IFN-g and TNFarb , but not TGF-b. These data demonstrate that regulatory mechanisms among human autologous T-cells can be mediated by cytolytic interactions or by the release of specific cytokines. Furthermore, they provide evidence that CD8 TCR-ab and TCR-gd cells differ in their patterns of recognition and in their abilities to modulate the immune response mediated by autologous autoreactive CD4 T-cells. q 1997 Elsevier Science B.V. |
| Starting Page | 47 |
| Ending Page | 64 |
| Page Count | 18 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0926-9851(97)00020-7 |
| Volume Number | 80 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0926985197000207 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0926985197000207?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0926-9851%2897%2900020-7 |
| Journal | Journal of Neuroimmunology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
