Loading...
Please wait, while we are loading the content...
Similar Documents
αβ T Cell Receptor Transfer to γδ T Cells Generates Functional Effector Cells without Mixed TCR Dimers In Vivo
| Content Provider | Scilit |
|---|---|
| Author | van der Veken, Lars T. Coccoris, Miriam Swart, Erwin Falkenburg, J. H. Frederik Schumacher, Ton N. Heemskerk, Mirjam H. M. |
| Copyright Year | 2009 |
| Description | The successful application of T cell-based immunotherapeutic applications depends on the availability of large numbers of T cells with the desired Ag specificity and phenotypic characteristics. Engineering of TCR-transferred T lymphocytes is an attractive strategy to obtain sufficient T cells with an Ag specificity of choice. However, the introduction of additional TCR chains into T cells leads to the generation of T cells with unknown specificity, due to the formation of mixed dimers between the endogenous and introduced TCR chains. The formation of such potentially autoaggressive T cells may be prevented by using γδ T cells as recipient cells, but the in vivo activity of such TCR-engineered γδ T cells has not been established. In the present study, we have investigated the in vivo functionality of TCR-transduced γδ T cells, in particular their Ag specific proliferative capacity, Ag specific reactivity, in vivo persistence, and their capacity to mount recall responses. The results demonstrate that αβ TCR engineering of γδ T cells forms a feasible strategy to generate Ag-specific effector T cells that do not express mixed TCR dimers. In view of increasing concerns on the potential autoimmune consequences of mixed TCR dimer formation, the testing of αβ TCR engineered γδ T cells in clinical trials seems warranted. |
| Ending Page | 170 |
| Page Count | 7 |
| Starting Page | 164 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.182.1.164 |
| Journal | The Journal of Immunology |
| Issue Number | 1 |
| Volume Number | 182 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2009-01-01 |
| Access Restriction | Open |
| Subject Keyword | Ag Specific Γδ T Cells Mixed Tcr Dimers |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
