NDLI: Novel dhps and pfcrt polymorphisms in Plasmodium falciparum detected by heteroduplex tracking assay.

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Novel dhps and pfcrt polymorphisms in Plasmodium falciparum detected by heteroduplex tracking assay.

Content Provider	Semantic Scholar
Author	Juliano, Jonathan J. Bacon, David J. Mu, Jianbing Wang, Xinhua Meshnick, Steven Richard
Copyright Year	2009
Abstract	Several single nucleotide polymorphisms (SNPs) have been linked to antimalarial drug resistance in Plasmodium falciparum. However, standard polymerase chain reaction (PCR) methods to detect these polymorphisms are unable to detect SNPs in variants representing < 20% of the parasites in a mixed infection, nor can they detect polymorphisms at nearby loci. Here we use heteroduplex tracking assays (HTAs) to analyze dhps540 mutations in 96 samples from Peru and pfcrt76 mutations in 70 samples from China. All samples had been previously analyzed by standard PCR. We detected drug-resistant minority variants and two novel non-synonymous pfcrt mutations in China. In Peru, we found no drug-resistant minority variants and a synonymous mutation in dhps. Thus, even in regions of low malaria transmission, HTA assays are more informative than PCR with agarose gel electrophoresis.
Starting Page	35
Ending Page	57
Page Count	23
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ajtmh.org/docserver/fulltext/14761645/80/5/0800734.pdf?accname=guest&checksum=497C92287EE697646A760782B351822B&expires=1541485903&id=id
PubMed reference number	19407115v1
Volume Number	80
Issue Number	5
Journal	The American journal of tropical medicine and hygiene
Language	English
Access Restriction	Open
Subject Keyword	Agarose Gel Antimalarials Coinfection Electrophoresis, Agar Gel Gel Electrophoresis (lab technique) Genetic Polymorphism HCCAT5 gene Malaria Nucleotides Parasites Plasmodium falciparum Polymerase Chain Reaction Silent Mutation Single Nucleotide Polymorphism Single-Chain Antibodies
Content Type	Text
Resource Type	Article

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