Loading...
Please wait, while we are loading the content...
Similar Documents
Analysis of Leptospira isolates from mainland Portugal and the Azores islands.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Collares-Pereira, M. J. Korver, Henri Santos-Reis, Margarida Bellenger, Emmanuel Baranton, Guy Terpstra, Wiepko J. |
| Copyright Year | 2000 |
| Abstract | From 228 recent Leptospira isolates from mainland Portugal and Azorean wild mammals, 149 were characterized at the serovar level by monoclonal antibodies (MAbs), a quick serological method in epidemiological studies. In order to compare this antigenic information with that from new genetic techniques, a sample of isolates was analyzed through pulsed-field agarose gel electrophoresis (PFGE) (n=71), mapped restriction site polymorphisms (MRSPs) in PCR-amplified rRNA genes (n=45, including 13 saprophytes) and arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting (n=32). MRSP and AP-PCR lead to species identification of the studied 32 pathogenic isolates: Leptospira interrogans (n=3), Leptospira kirschneri (n=8) and Leptospira borgpetersenii (n=21). MAbs and PFGE characterized pathogenic isolates at the serovar level and resulted mainly in agreement (64%) although many discrepancies (35%) were observed. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://watermark.silverchair.com/185-2-181.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbowggG2BgkqhkiG9w0BBwagggGnMIIBowIBADCCAZwGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMeUPDtnJqIbRYQjQyAgEQgIIBbbRpz-4m9u7OENXNyR2A17URA-vtNW4RQlI3Si0uFUi7RcC73MZs0wlR6dvL0pBr5Ir6QT1kHKZPR5PNV0GwTqG6XEsHm8V--iN-vwNRQjXcDjM_FITVMP8wgqgpki2E-ltjgWLbMbe6YFh-x9CWieciPZMLqW1uATRWVopmCSZkskLQ2R8LZLeuDa2K2YnqI8ky4N2t3ux5Kq76roHcnWp1H48q_IZC0bMvDWcQpzmo_gokJreet6K1xvKqDSmSPMV9J3e93ft4Q1GPirSXDI0LMDHxYEfus3NUOuyuA9jZfSjEEK8tXbMBz2R_emDe-5Fho8w5jcKwzh3Octo6xGN6YIzTrodbtofAcDN-FbHOX31pJkye0vD4QRAPNUutq7T-OkqwDXIylgV9xOAtRyE8kWPdCUnZzXYMDcZpkOUDt9KQBkmDVcm4K02ESDmi0mFPX2W3ohyBKVD6tXqDy7liTmi39Wy-wL8RC5yu |
| PubMed reference number | 10754245v1 |
| Volume Number | 185 |
| Issue Number | 2 |
| Journal | FEMS microbiology letters |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Agarose Gel Electrophoresis, Agar Gel Epidemiology Gel Electrophoresis (lab technique) Genetic Polymorphism Genetic Techniques Leptospira interrogans Mammals Polymerase Chain Reaction Serotype |
| Content Type | Text |
| Resource Type | Article |
