NDLI: Study of cynomolgus monkey (Macaca fascicularis) Mhc DRB gene polymorphism in four populations

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Study of cynomolgus monkey (Macaca fascicularis) Mhc DRB gene polymorphism in four populations

Content Provider	Semantic Scholar
Author	Blancher, Antoine Aarnink, Alice Tanaka, Keiko Ota, Masao Inoko, Hidetoshi Yamanaka, Hisashi Nakagawa, Hiroshi Apoil, P. A. Shiina, Takashi
Copyright Year	2012
Abstract	The cynomolgus macaque (Macaca fascicularis) is currently used as an animal model in various fields of immunology especially in the development of innovative vaccines for the prevention and treatment of infectious diseases. The polymorphism of the major histocompatibility complex (MHC) influences the development of adaptive immune responses, and it is crucial to characterize the polymorphism of cynomolgus MHC genes. Among all macaque species, the cynomolgus macaque has the most diversified geographical area encompassing continental and insular populations. By the study of a large sample of animals from the Philippines (N = 359), we have characterized 20 DRB haplotypes. The DRB genotyping was performed by denaturing gradient gel electrophoresis (DGGE) sequencing of exon 2 and was confirmed by polymerase chain reaction–sequence-specific oligonucleotide. The DRB and DRA cDNA of 126 animals were characterized by cloning and sequencing. By means of DGGE sequencing, we characterized the polymorphism of genomic DRB exon 2 in three other cynomolgus macaque population samples (Java, Vietnam, and Mauritius), and we discuss about the origin of the founders of the Mauritian and the Filipino cynomolgus macaque populations.
Starting Page	605
Ending Page	614
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1007/s00251-012-0613-5
Alternate Webpage(s)	https://static-content.springer.com/esm/art:10.1007%2Fs00251-012-0613-5/MediaObjects/251_2012_613_MOESM1_ESM.pdf
PubMed reference number	22790512
Alternate Webpage(s)	https://doi.org/10.1007/s00251-012-0613-5
Journal	Medline
Volume Number	64
Journal	Immunogenetics
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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