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Produção de biossurfactante por consórcio microbiano e aplicação na remediação de solo contaminado com arsênio.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Araújo, Larissa Dos Santos |
| Copyright Year | 2019 |
| Abstract | Biosurfactants are amphiphilic biomolecules with surface-active properties, synthesized by organisms such as bacteria, fungi and plants under different conditions and nutritional sources. These molecules are widely used by industry, including the environmental applications as soil remediation. Diverse species of microorganisms are able to produce biosurfactants in the environment and are usually in communities or consortia. This work aimed the study the biosurfactant production by a microbial consortium and evaluation of the efficiency of the extracts in the remediation of As in soil from Mine Chico Rei, located in Ouro Preto, Brazil. Firstly, the microorganisms’ constituents of the consortium were identified using the Ion Torrent® technique, and the optimum conditions (COP) for biosurfactant production were established. The optimum condition was assessed in order to obtain the higher surface–activity measured by surface tension reduction and emulsification index. In addition, the influence of 0.5, 1.0, 1.5 and 2.5 ratios of glucose and glycerol in the production were also assessed. Aiming the mobilization of As in a Fe rich soil, sequential extractions were conducted for evaluation of the extracts with different ratio glu/gly and COP in pH 3, 7 and 11 and compared to the distilled water, SDS 1% and saponin 0,1%. The toxicity of the leaching effluent was evaluated with Allium cepa seeds. The Pseudomonas and Stenotrophomonas genus were indicated as the main genus present in the consortium. The higher emulsification index was obtained for the extract from the culture grown in ratio glucose/glycerol 2,5, pH 9,5, after three days of incubation at 25°C. The efficiency of the As extraction by the BS was superior for all the glu/gly ratios extracts compared to the other solutions evaluated. The higher As extraction efficiency (24,6%) was obtained for COP at pH 11 (678,39 mg.kg-1) in 7 cycles of 24 hours. The presence of As affected the germination and the growth of the Allium cepa seedlings negatively. The concentration of As extracted and the height of leaves and stalk were inversely correlated (R2=95,89%). The As attenuation was verified by an ecotoxicological assay and viability of reuse of the remediated soil in mixtures of 20, 50 and 80% with the vegetal substrate were investigated, and only the most diluted mixtures of treated soil can be potentially reutilized for landscaping with the contention of the reminiscent irrigation water |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.repositorio.ufop.br/bitstream/123456789/11824/1/DISSERTA%C3%87%C3%83O_Produ%C3%A7%C3%A3oBiossurfactanteCons%C3%B3rcio.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
