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Gold nanoparticle sensitize radiotherapy of prostate cancer cells by regulation of the cell cycle.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Roa, Wilson H.-Y. Zhang, Xiaojing Guo, Linghong Sterling, Heidi Hu, Xiuying Xiong, Yeping Gulavita, Sunil P. P. Patel, Samir Sun, Xuejun Chen, Jie Moore, Ronald M. Xing, James |
| Copyright Year | 2009 |
| Abstract | Glucose-capped gold nanoparticles (Glu-GNPs) have been used to improve cellular targeting and radio-sensitization. In this study, we explored the mechanism of Glu-GNP enhanced radiation sensitivity in radiation-resistant human prostate cancer cells. Cell survival and proliferation were measured using MTT and clonogenic assay. Flow cytometry with staining by propidium iodide (PI) was performed to study the cell cycle changes induced by Glu-GNPs, and western blotting was used to determine the expression of p53 and cyclin proteins that correlated to cell cycle regulation. With 2 Gy of ortho-voltage irradiation, Glu-GNP showed a 1.5-2.0 fold enhancement in growth inhibition when compared to x-rays alone. Comparing the cell cycle change, Glu-GNPs induced acceleration in the G0/G1 phase and accumulation of cells in the G2/M phase at 29.8% versus 18.4% for controls at 24 h. G2/M arrest was accompanied by decreased expression of p53 and cyclin A, and increased expression of cyclin B1 and cyclin E. In conclusion, Glu-GNPs trigger activation of the CDK kinases leading to cell cycle acceleration in the G0/G1 phase and accumulation in the G2/M phase. This activation is accompanied by a striking sensitization to ionizing radiation, which may have clinical implications. |
| Starting Page | 375101 |
| Ending Page | 375101 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://binaryrg.ece.ualberta.ca/images/manuscript/publications/2009/2009-43.pdf |
| PubMed reference number | 19706948v1 |
| Alternate Webpage(s) | https://doi.org/10.1088/0957-4484/20/37/375101 |
| DOI | 10.1088/0957-4484/20/37/375101 |
| Journal | Nanotechnology |
| Volume Number | 20 |
| Issue Number | 37 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Artificial nanoparticles CDK6-associated protein p18 Cardiac Arrest Cell Cycle Control Cell Survival Cyclin B1 Cyclins Glucose Glutamic Acid Iodides Ionizing radiation Mitosis Propidium Iodide Prostatic Neoplasms Radiation Tolerance Roentgen Rays Sensitization (observable entity) Staining method Western Blotting cellular targeting monooxyethylene trimethylolpropane tristearate voltage |
| Content Type | Text |
| Resource Type | Article |
