Loading...
Please wait, while we are loading the content...
Similar Documents
Downregulation of NIMA-related kinase-7 inhibits cell proliferation by inducing cell cycle arrest in human retinoblastoma cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhang, Jian Wang, Liqian Zhang, Yongkang |
| Copyright Year | 2017 |
| Abstract | NIMA-related kinase-7 (Nek7) is a centrosomal kinase involved in various types of cancer, including gallbladder cancer and hepatocellular carcinoma. However, the biological function and the potential underlying mechanism of Nek7 in retinoblastoma remain largely unknown. Therefore, the present study investigated the effects of Nek7 in retinoblastoma cells. The expression of Nek7 was initially determined and observed to be commonly upregulated in retinoblastoma cell lines (Y79, SO-RB50 and WERI-RB1) as compared with that in normal retinal pigment epithelium cells. Next, the endogenous expression of Nek7 was efficiently knocked down in Y79 and SO-RB50 cells using a lentivirus-mediated RNA interference approach, as confirmed by reverse transcription-quantitative polymerase chain reaction and western blot analysis. Loss-of-function assays, including MTT, colony formation and flow cytometry, indicated that knockdown of Nek7 significantly inhibited cell growth, impaired the colony formation ability and induced cell cycle arrest at G0/G1 phase. Furthermore, mechanistic studies demonstrated that silencing of Nek7 resulted in reduced cyclin-dependent kinase 2, cyclin D1 and cyclin E levels in vitro. In conclusion, the present study highlights the crucial role of Nek7 in promoting retinoblastoma cell proliferation, and Nek7-silencing may serve as a novel therapeutic target for retinoblastoma. |
| Starting Page | 1360 |
| Ending Page | 1366 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 29434721v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/etm.2017.5558 |
| DOI | 10.3892/etm.2017.5558 |
| Journal | Experimental and therapeutic medicine |
| Volume Number | 15 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CDK2 gene Cell Cycle Arrest Cell Proliferation Cyclin-Dependent Kinases Cyclins Flow Cytometry Gallbladder Carcinoma Liver carcinoma Negative Regulation of Cell Cycle Polymerase Chain Reaction Promotion (action) RNA Interference Retina Retinal Pigments Retinoblastoma Reverse Transcription Structure of retinal pigment epithelium Western Blotting cell growth monooxyethylene trimethylolpropane tristearate nima |
| Content Type | Text |
| Resource Type | Article |
