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Hsa-miR-429 promotes bladder cancer cell proliferation via inhibiting CDKN2B
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yang, Jiangeng Liu, Yuchen He, Anbang Liu, Yuhan Helena Wu, Jianting Liao, Xinhui Lv, Zhaojie Wang, Feng Mei, Hongbing |
| Copyright Year | 2017 |
| Abstract | BACKGROUND AND OBJECTIVES Hsa-miR-429 is increased in bladder cancer. Its roles in bladder cancer are poorly understood. METHODS The expression levels of hsa-miR-429 and cyclin-dependent kinase inhibitor 2B (CDKN2B) were determined using Real-Time qPCR in a total of 50 patients with bladder cancer. Bladder cancer T24 and 5637 cells were transfected CDKN2B siRNA or hsa-miR-429 mimic. CDKN2B expression levels after transfection were detected by Real-Time qPCR and Western blot assay respectively. Binding sites between hsa-miR-429 and 3'-untranslated region of CDKN2B were confirmed by Dual luciferase reporter assay. Cell proliferation was evaluated using MTT and EdU assays. Cell apoptosis was determined using ELISA assay. RESULTS Higher hsa-miR-429 expression levels were associated with higher tumor grade and stage. All patients with low hsa-miR-429 expression survived 5 years, while with high hsa-miR-429 expression, only 58% survived. Hsa-miR-429 and CDKN2B were inversely expressed in bladder cancer. Hsa-miR-429 mimic decreased the expression of CDKN2B at both mRNA and protein levels. The binding site was confirmed between hsa-miR-429 and 3'-untranslated region of CDKN2B. Up-regulation of hsa-miR-429 or down-regulation of CDKN2B promoted cell growth and decreased apoptosis. CONCLUSIONS Our data suggest a mechanism for hsa-miR-429 to play oncogenic roles via inhibiting CDKN2B. |
| Starting Page | 68721 |
| Ending Page | 68729 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&op=download&page=article&path%5B%5D=19878&path%5B%5D=63445 |
| PubMed reference number | 28978151v1 |
| Alternate Webpage(s) | https://doi.org/10.18632/oncotarget.19878 |
| DOI | 10.18632/oncotarget.19878 |
| Journal | Oncotarget |
| Volume Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Binding Sites Bladder Neoplasm CDK6-associated protein p18 Cell Proliferation Cyclin-Dependent Kinases Cyclins Down-Regulation Inhibition Ligand Binding Domain Malignant neoplasm of urinary bladder Neoplasms Patients Western Blot cancer cell cell growth ethylene diurea monooxyethylene trimethylolpropane tristearate mts2 endopeptidase protein |
| Content Type | Text |
| Resource Type | Article |
