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Increased secretion of urokinase-type plasminogen activator by human lung microvascular endothelial cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Takahashi, Keiko F. Uwabe, Yasuhide Sawasaki, Yoshio Kiguchi, Toshio Nakamura, Hidetoshi Kashiwabara, Kenji Yagyu, Hiroaki Matsuoka, Takeshi |
| Copyright Year | 1998 |
| Abstract | Human lung microvascular endothelial cells (HLMECs) secreted 1.5-15 times more urokinase-type plasminogen activator (uPA) antigen than human hepatic microvascular endothelial cells, human umbilical vein endothelial cells (HUVECs), angioma endothelial cells, and lung fibroblasts. All of these cells also secreted a 100-fold greater amount of plasminogen activator inhibitor-1 than of uPA antigen, and uPA activities were not detected in the culture medium. The expression of uPA mRNA in HLMECs was higher (100-fold) compared with HUVECs, angioma endothelial cells, and lung fibroblasts. HLMECs secreted uPA antigen on both the luminal and basal sides of the cells. On the other hand, HLMECs secreted a 10- to 15-fold lower amount of tissue-type plasminogen activator than HUVECs, mostly on the luminal side. After stimulation with interleukin (IL)-1beta, HLMECs secreted a six- to ninefold amount of uPA antigen. In contrast, no stimulatory effect was observed in HUVECs even under high IL-1beta concentrations. The secretion of uPA and plasminogen activator inhibitor-1 from HLMECs was also enhanced by tumor necrosis factor-alpha and IL-2. These results suggest that HLMECs may contribute not only to the patency of lung vessels but also to the maintenance of alveolar functions through the production and secretion of uPA, especially in the presence of inflammatory cytokines. |
| Starting Page | 1 |
| Ending Page | 2 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ajplung.physiology.org/content/ajplung/275/1/l47.full.pdf |
| PubMed reference number | 9688934v1 |
| Volume Number | 275 |
| Issue Number | 1 |
| Part | 1 |
| Journal | The American journal of physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blood Vessel Cell secretion Culture Media Hemangioma Human Umbilical Vein Endothelial Cells Interleukin-1 beta Interleukins Phenobarbital Plasminogen Activator SERPINE1 protein, human Structure of parenchyma of lung Tumor Necrosis Factors Umbilicus (Anatomy) Urokinase alteplase |
| Content Type | Text |
| Resource Type | Article |
