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VEGF-C mediates cyclic pressure-induced endothelial cell proliferation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shin, Hainsworth Y. Smith, Michael L. Toy, Karen J. Williams, P. Mickey Bizios, Rena Gerritsen, Mary E. |
| Copyright Year | 2002 |
| Abstract | Mechanical forces modulate endothelial cell functions through several mechanisms including regulation of gene transcription. In the present study, gene transcription by human umbilical vein endothelial cells (HUVEC) either maintained under control pressure (that is, standard cell culture conditions equivalent to 0.15 mmHg sustained hydrostatic pressure) or exposed to 60/20 mmHg sinusoidal pressures at 1 Hz were compared using Affymetrix GeneChip microarrays to identify cellular/molecular mechanisms associated with endothelial cell responses to cyclic pressure. Cyclic pressure selectively affected transcription of 14 genes that included a set of mechanosensitive proteins involved in hemostasis (tissue plasminogen activator), cell adhesion (integrin-alpha2), and cell signaling (Rho B, cytosolic phospholipase A2), as well as a unique subset of cyclic pressure-sensitive genes such as vascular endothelial growth factor (VEGF)-C and transforming growth factor (TGF)-beta2. The present study also provided first evidence that VEGF-C, the most highly induced gene under 60/20 mmHg, mediated HUVEC proliferation in response to this cyclic pressure. Cyclic pressure is, therefore, a mechanical force that modulates endothelial cell functions (such as proliferation) by activating a specific transcriptional program. |
| Starting Page | 1716 |
| Ending Page | 1719 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://physiolgenomics.physiology.org/content/physiolgenomics/11/3/245.full.pdf |
| PubMed reference number | 12388793v1 |
| Volume Number | 11 |
| Issue Number | 3 |
| Journal | Physiological genomics |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affymetrix Cell Adhesion Cell Culture Techniques Cell Proliferation Cell physiology Growth Factor Hemostatic function Human Umbilical Vein Endothelial Cells Hydrostatic Pressure PLAT protein, human Phospholipase Plasminogen Activator Subgroup Transcription, Genetic Transforming Growth Factors Umbilicus (Anatomy) alteplase torr |
| Content Type | Text |
| Resource Type | Article |
