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Close association between interleukin 2 receptor mRNA expression and human T cell leukemia/lymphoma virus type I viral RNA expression in short-term cultured leukemic cells from adult T cell leukemia patients.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Umadome, Hiroshi Uchiyama, Takashi Hori, Toshiyuki Tamori, S. Motoi, Tomomi Araki, Kimi Uchino, Haruto |
| Copyright Year | 1988 |
| Abstract | Human T cell leukemia/lymphoma (T-lymphotropic) virus type I (HTLV-I) infection has been considered to be closely associated with the leukemogenesis of adult T cell leukemia (ATL), in which interleukin 2 (IL-2) receptors are abnormally expressed. In this study, however, Southern blot analysis revealed no gross rearrangement or obvious amplification of the IL-2 receptor gene in ATL leukemic cells, indicating that abnormal IL-2 receptor expression in ATL is not due to the structural change of its gene. Hence, we studied the expression of the IL-2 receptor and HTLV-I at the RNA level during short-term cultures of leukemic cells from 9 ATL patients. Cytoplasmic dot hybridization and Northern hybridization revealed that fresh leukemic cells from seven of nine patients expressed a small amount of IL-2 receptor mRNA but HTLV-I RNA was undetectable in all cases. After cultures for up to 7 d, both IL-2 receptor mRNA and HTLV-I RNA (including pX message) expression concomitantly increased, whereas the amounts of other cellular genes, except for beta-actin, did not. The increases in their RNA expression were inhibited by early addition (within 12 h after the beginning of the culture) of cycloheximide, indicating that these increases are mediated by newly synthesized protein(s). These results strongly suggested that IL-2 receptor expression is closely associated with HTLV-I expression in leukemic cells from ATL patients. |
| Starting Page | 52 |
| Ending Page | 61 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dm5migu4zj3pb.cloudfront.net/manuscripts/113000/113309/JCI88113309.pdf |
| PubMed reference number | 2891729v1 |
| Volume Number | 81 |
| Issue Number | 1 |
| Journal | The Journal of clinical investigation |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | ACTB gene Adult T-Cell Lymphoma/Leukemia Cycloheximide DNA Sequence Rearrangement Feline Immunodeficiency Virus Human T-lymphotropic virus 1 Interleukin 2 Receptor Interleukin-2 Leukemia, B-Cell Leukemia, T-Cell Leukemogenesis Lymphoma Nucleic Acid Hybridization Patients RNA Recombinant Interleukins T-Lymphocyte Transcription, Genetic leukemia viral transcription |
| Content Type | Text |
| Resource Type | Article |
